Applications are invited for a Postdoctoral Research Scientist to join the Laboratory of Conrad Nieduszynski at the Earlham Institute, based in Norwich, UK.
The role of Senataxins in regulating replication-transcription clashes
Complete, accurate genome replication is essential for life. However, a major potential obstacle to the replication apparatus is transcription, particularly in cancerous cells (Gómez-González & Aguilera, 2019). Although cells possess efficient pathways that process transcription-replication conflicts, these protective mechanisms are not well understood. This project will discover how Senataxins contribute to the resolution of transcription-replication conflicts.
In collaboration with the Carr group (Genome Damage and Stability Centre, University of Sussex), we will use a powerful collection of yeast mutants to explore the function of Senataxins. We will apply cutting-edge single-molecule nanopore sequencing techniques, combined with classical yeast genetics and cell biology, to track the movement of the replication machinery on individual DNA molecules from single cells (Müller et al., 2019). This will allow us to understand the causes of transcription-replication conflicts and how they are resolved by Senataxin.
Gómez-González & Aguilera (2019). Transcription-mediated replication hindrance: a major driver of genome instability. Gene Dev, 33: 1008.
Müller et al. (2019). Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads. Nat. Methods,16:429.
This post requires a molecular/genome biologist with experience of high throughput sequencing with an interest in using their expertise to study fundamental biological processes and the causes of genome instability. The post-holder will be involved in yeast genetics, molecular biology and single molecule high-throughput sequencing.
The ideal candidate:
The successful candidate will be a trained, enthusiastic and self-motivated scientist with experience in applying high-throughput sequencing in scientific research. The post-holder should hold a PhD/DPhil in molecular biology, biochemistry, genomics, genetics or equivalent. Previous experience researching, developing and optimising molecular biology protocols is essential. Furthermore, good organisational skills and the ability to communicate and work productively within a multidisciplinary team will be crucial. An interest in chromosome biology will be essential, but prior experience or knowledge of DNA replication or genome stability is not a requirement. Candidates with a combination of molecular biology and bioinformatics experience would be especially welcome. Training will be available in all aspects of the work, although it is expected that applicants will have a strong background in the majority of the molecular and genomic techniques.
Salary on appointment will be within the range £33,050 to £40,750 per annum depending on qualifications and experience. This is a full-time post for a contract of 3 years.
Interviews will be held on 11th August 2022.
For further information and details of how to apply, please visit our website http://jobs.earlham.ac.uk/ or contact the Human Resources team on 01603 450462 or [email protected] quoting reference 1004293.
As a Disability Confident employer, we guarantee to offer an interview to all disabled applicants who meet the essential criteria for this vacancy.
The closing date for applications will be 31st July 2022.